Spiked GBS: a new marker platform

New paper in BMC Genomics that presents spiked GBS, which combines targeted amplicon sequencing with reduced representation genotyping-by-sequencing. To reduce the cost of targeted assays, we use a small percent of sequencing capacity available in runs of GBS libraries to “spike” amplified targets tagged with a different set of unique barcodes. This open platform will allow multiple, single-target loci to be assayed while simultaneously generating a whole-genome profile.